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A convenient synthesis of N-succinimidyl-3-iodo-[125I]benzoate, a reagent for protein iodination
1Department of Radiochemistry, Nuclear Research Center-Negev, Beer-Sheva, Israel.
International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
|August 1, 1992
Abstract:
A convenient procedure has been developed for the synthesis of N-succinimidyl-3-iodo-[125I]benzoate. The procedure involved the synthesis of chloromercuribenzoic acid, its esterification with N-hydroxysuccinimide (NHS) and exchanging the mercury moiety with radioactive iodine in the presence of an oxidant. The obtained product was attached to human serum albumin and its stability was compared with Chloramine-T (Ch-T) radioiodinated protein. The results indicated that the reagent-radiolabeled protein was stable for longer periods and the deiodination rate was significantly lower.