Two-photon confocal microscopy: a nondestructive method for studying wound healing

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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

Fernando A Navarro1, Peter T C So, Rubin Nirmalan

  • 1Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA 02115, USA.

Plastic and Reconstructive Surgery
|June 29, 2004
PubMed

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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...