Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oligopeptidase A is required for normal phage P22 development.

C A Conlin1, E R Vimr, C G Miller

  • 1Department of Microbiology, University of Illinois, Urbana-Champaign 61801.

Journal of Bacteriology
|September 1, 1992
PubMed
Summary

Salmonella typhimurium oligopeptidase A (OpdA) is essential for bacteriophage P22 particle maturation. OpdA processes phage protein gp7, crucial for DNA injection, enabling efficient phage production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A double-blind, randomized, Phase III, multicenter study in 358 pediatric subjects receiving isotretinoin therapy demonstrates no effect on pediatric bone mineral density.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

OARSI Clinical Trials Recommendations: Knee imaging in clinical trials in osteoarthritis.

Osteoarthritis and cartilage·2015
Same author

OARSI Clinical Trials Recommendations: Hip imaging in clinical trials in osteoarthritis.

Osteoarthritis and cartilage·2015
Same author

OARSI Clinical Trials Recommendations: Hand imaging in clinical trials in osteoarthritis.

Osteoarthritis and cartilage·2015
Same author

Imaging atlas for eligibility and on-study safety of potential knee adverse events in anti-NGF studies (Part 1).

Osteoarthritis and cartilage·2014
Same author

The current status of imaging in anti-NGF clinical trials.

Osteoarthritis and cartilage·2014

Area of Science:

  • Microbiology
  • Virology
  • Molecular Biology

Background:

  • The opdA gene in Salmonella typhimurium encodes oligopeptidase A (OpdA), an endoprotease.
  • Bacteriophage P22 infection efficiency is impacted by host opdA mutations.

Purpose of the Study:

  • To investigate the role of Salmonella typhimurium oligopeptidase A (OpdA) in bacteriophage P22 development.
  • To identify the specific target of OpdA action during phage P22 replication.

Main Methods:

  • Isolation and characterization of P22 mutants resistant to opdA deficiency.
  • Marker rescue experiments and DNA sequencing to map mutations.
  • Analysis of phage protein processing and particle infectivity.

Main Results:

Related Experiment Videos

  • opdA mutant hosts produced significantly fewer infectious P22 particles due to defective particle formation.
  • Mutations conferring opdA independence were localized to the N-terminal region of P22 gene 7 (gp7).
  • OpdA-dependent processing of a 20-amino-acid peptide from gp7 was identified as essential for P22 DNA injection.

Conclusions:

  • Oligopeptidase A (OpdA) plays a critical role in the maturation of bacteriophage P22 by processing the essential DNA injection protein gp7.
  • Understanding this interaction provides insights into phage-host dynamics and phage assembly mechanisms.