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Related Experiment Videos

Genetic diversity among sapoviruses.

T Farkas1, W M Zhong, Y Jing

  • 1Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA. Tibor.Farkas@cchmc.org

Archives of Virology
|June 29, 2004
PubMed
Summary

This study reveals significant genetic diversity within sapoviruses (SAP), identifying potential new clusters and genogroups. These findings enhance our understanding of SAP classification and epidemiology.

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Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Norovirus and Sapovirus cause acute gastroenteritis.
  • Noroviruses are genetically diverse, but Sapovirus diversity is less understood.
  • Characterizing Sapovirus genetic diversity is crucial for understanding its epidemiology.

Purpose of the Study:

  • To genetically characterize 25 Sapovirus strains from human gastroenteritis outbreaks and cases.
  • To investigate the genetic diversity of Sapovirus using RNA polymerase and capsid gene sequences.
  • To propose an updated classification of Sapovirus based on genetic analysis.

Main Methods:

  • Sequencing of partial RNA polymerase and capsid genes from 25 Sapovirus strains.
  • Phylogenetic and distance analyses to group strains and identify genetic relationships.

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  • Comparison of sequences with existing GenBank data.

    • Main Results:

    • The 25 Sapovirus strains grouped into 12 genetic clusters based on RNA polymerase sequences, with 9 potential new clusters identified.
    • Capsid gene analysis supported the grouping and revealed four strains with <84% amino acid identity to known sequences.
    • Phylogenetic analysis indicated two new genogroups and two new clusters within an existing genogroup, suggesting a revised classification.

    Conclusions:

    • Sapoviruses exhibit significant genetic diversity, necessitating further research.
    • The study proposes a classification of Sapoviruses into nine genetic clusters within five genogroups.
    • This revised classification includes porcine enteric calicivirus (PEC) and aids in understanding Sapovirus evolution and epidemiology.