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Simultaneous purification of the neuroproteins synapsin I and synaptophysin
I Llona1, W G Annaert, W P De Potter
1Department of Medicine, University of Antwerp (UIA), Wilrijk, Belgium.
Journal of Chromatography
|April 3, 1992
Abstract:
A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40 micrograms/g in brain for synaptophysin and 25 micrograms/g in brain for synapsin I.