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Updated: Jul 22, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural
Matthew D Simon1, Kevan M Shokat
1Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Abstract:
We explore the adaptability at a protein-DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.
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