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Updated: Aug 23, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
[Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen]
Yi-ping Xing1, Zu-hu Huang, Shi-xia Wang
1Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Objectives:
To observe immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen (HBcAg).
Methods:
A new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen was constructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice were immunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linked immune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH release assay.
Results:
pSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showed strong anti-HBc response and specific high cytotoxicity of CTL.
Conclusion:
pSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice.
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