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Updated: Aug 23, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
Published on: December 13, 2019
Detection of DNA hybridization on a liposome surface using ultrasound velocimetry and turbidimetry methods
Tibor Hianik1, Peter Rybar, Sergej Yu Andreev
1Department of Biophysics and Chemical Physics, Comenius University, 842 48 Bratislava, Slovakia. Hianik@fmph.uniba.sk
Abstract:
19-mer oligonucleotides with oleylamine tethered at 3' and 5' terminal, respectively, were incorporated into unilamellar liposomes of dioleoylphosphatidylcholine (DOPC). Addition of complementary nucleotide resulted in hybridization with oligonucleotides located on different liposomes and caused liposome aggregation. Significant changes of sound velocimetry and turbidity were readily observed at 10 nM concentration of the complementary chain.
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