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Flow cytometric screening of aldolase catalytic antibodies
Hyunbo Shim1, Amelie Karlström, Sofia M Touami
1The Skaggs Institute for Chemical Biology and Departments of Molecular Biology and Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Bioorganic & Medicinal Chemistry Letters
|July 1, 2004
Abstract:
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
