Related Experiment Video
Updated: Aug 8, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
Intraperitoneal and intraluminal microdialysis in the detection of experimental regional intestinal ischaemia
1Department of Surgical Gastroenterology, Aalborg University Hospital, Aalborg, Denmark. sommers@privat.dk
Background:
The aim was to investigate the possibility of using intraluminal or intraperitoneal microdialysis to monitor regional intestinal ischaemia.
Methods:
Microdialysis catheters were inserted in the lumen, in and outside the intestinal wall, and in the peritoneum of each of ten pigs. Regional occlusive ischaemia was induced in 100 cm of jejunum. Levels of glucose, pyruvate, lactate and glycerol in the microdialysate were measured at 20-min intervals before and after induction of ischaemia. Systemic haemodynamics were monitored and laser Doppler flowmetry (LDF) recordings made in each of the intestinal segments.
Results:
Ischaemia caused a significant decrease in glucose level, and an increase in lactate and glycerol concentrations and lactate/pyruvate ratio, at all catheters, although glucose could not be detected by the intraluminal catheter. The metabolic changes occurred simultaneously and were statistically significant in almost all catheters after 100 min. LDF revealed a significant decrease in intestinal blood flow, but there was considerable individual variation.
Conclusion:
Regional occlusive ischaemia in 100 cm of small intestine could be detected and monitored by means of a microdialysis catheter placed in the peritoneal cavity or the bowel lumen.
