Design and synthesis of a solid-supported FR225659 derivative for its receptor screening
Tatsuya Zenkoh1, Hidetaka Hatori, Hiroshi Tanaka
1Department of Applied Chemistry, Tokyo Institute of Technology, Meguro, Tokyo 152-8552, Japan.
Organic Letters
|July 2, 2004
Abstract:
[structure: see text] We describe the design and synthesis of latex particles attached to an FR225659 derivative to identify its receptor proteins. Two key building blocks were prepared by two-step degradation of FR225659 under basic conditions. The designed ligand showed an acceptable level of biological activity to make it of potential value for use in affinity-supported receptor identification. Affinity purification of FR225659-binding proteins using the latex nanoparticles provided three candidate receptor peptides for the biological activity.


