Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA displacement assay integrated into microfluidic channels.

Rebecca A Zangmeister1, Michael J Tarlov

  • 1Chemical Science and Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA. razang@nist.gov

Analytical Chemistry
|July 2, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recommended nomenclature convention for the NISTCHO cell line and its product monoclonal antibody, cNISTmAb.

mAbs·2025
Same author

Absolute quantification method for protein concentration.

Analytical chemistry·2014
Same author

Characterization of polydopamine thin films deposited at short times by autoxidation of dopamine.

Langmuir : the ACS journal of surfaces and colloids·2013
Same author

Competitive adsorption-desorption of IgM monomers-dimers on silica and modified silica surfaces.

Journal of colloid and interface science·2013
Same author

Quantifying ligand adsorption to nanoparticles using tandem differential mobility mass analysis.

Analytical chemistry·2012
Same author

Protein adsorption-desorption on electrospray capillary walls--no influence on aggregate distribution.

Journal of colloid and interface science·2012

This study introduces a novel microfluidic assay for DNA diagnostics. The innovative method detects unlabeled DNA sequences quickly using fluorescence, improving diagnostic speed and accessibility.

Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Analytical Chemistry

Background:

  • Traditional DNA analysis often requires time-consuming labeling steps.
  • Microfluidic devices offer miniaturized platforms for rapid biological assays.
  • Developing label-free detection methods is crucial for efficient diagnostics.

Purpose of the Study:

  • To develop a novel fluorescence-based microfluidic assay for DNA diagnostics.
  • To enable the detection of unlabeled target DNA sequences.
  • To reduce assay times through directed electrophoretic transport.

Main Methods:

  • Development of a microfluidic channel with an immobilized DNA-containing hydrogel plug.
  • Utilizing a sacrificial fluorescent-tagged indicator oligomer.

Related Experiment Videos

  • Employing electrophoretic transport to move target DNA towards the sensing element.
  • Monitoring the displacement of the indicator oligomer by the target sequence.

    • Main Results:

    • Successful development of a label-free fluorescence-based DNA diagnostic assay.
    • Demonstration of target DNA sequence detection without prior labeling.
    • Achieved short sensing times due to rapid electrophoretic transport.
    • The assay relies on the displacement principle for signal generation.

    Conclusions:

    • The developed microfluidic assay offers a unique and efficient method for DNA diagnostics.
    • The label-free approach significantly simplifies the diagnostic workflow.
    • This technology holds promise for faster and more accessible DNA analysis.