Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A soft lithographic approach to fabricate patterned microfluidic channels.

Ali Khademhosseini1, Kahp Y Suh, Sangyong Jon

  • 1Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E25-342, Cambridge, MA 02139, USA.

Analytical Chemistry
|July 2, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ three-dimensional printing for reparative and regenerative therapy.

Biomedical microdevices·2019
Same author

A Perspective on 3D Bioprinting in Tissue Regeneration.

Bio-design and manufacturing·2019
Same author

Sutureless repair of corneal injuries using naturally derived bioadhesive hydrogels.

Science advances·2019
Same author

Hierarchically Patterned Polydopamine-Containing Membranes for Periodontal Tissue Engineering.

ACS nano·2019
Same author

A Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening.

Small (Weinheim an der Bergstrasse, Germany)·2019
Same author

A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication·2019

Researchers developed a new microfluidic technique for precise surface patterning. This method enables controlled cell and protein placement within microchannels, advancing diagnostics and cell biology studies.

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Precise control over surface properties and molecular presentation in microfluidic channels is crucial for developing diagnostic assays, microreactors, and for fundamental studies in cell biology and fluid mechanics.
  • Existing soft lithography methods require improved techniques for fabricating robust microchannels with enhanced spatial control over substrates.

Purpose of the Study:

  • To present a simple, broadly applicable soft lithography technique for fabricating microchannels with precise spatial control over surface properties.
  • To demonstrate the patterning of nonbiofouling molecules and biological entities within these microchannels.
  • To showcase the potential for advanced applications such as multi-protein deposition and cell-based assays.

Main Methods:

Related Experiment Videos

  • A novel technique involving the use of poly(dimethylsiloxane) (PDMS) stamps to protect patterned regions from oxygen plasma treatment during microchannel fabrication.
  • Irreversible bonding of the microfluidic mold to the substrate after plasma treatment and PDMS stamp removal.
  • Application of laminar flow for controlled deposition of multiple proteins onto patterned surfaces.

    • Main Results:

    • Fabrication of robust microchannels with precise spatial control over substrate properties.
    • Successful patterning of nonbiofouling poly(ethylene glycol)-based copolymer and hyaluronic acid within microchannels.
    • Creation of patterned arrays of fibronectin, bovine serum albumin, and viable mammalian cells.
    • Demonstrated control over multi-protein deposition using laminar flow.
    • Confirmed viability and intracellular activity of patterned cells, with potential for subsequent lysis and analysis.

    Conclusions:

    • The presented technique offers a simple and effective method for fabricating microfluidic devices with spatially controlled surface properties.
    • This approach enables the precise patterning of various molecules and cells, facilitating advanced applications in diagnostics, cell biology, and microfluidics.
    • The method ensures cell viability and functionality within the microchannels, opening avenues for complex cellular assays and analyses.