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Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
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Protein bead array for the detection of HIV-1 antibodies from fresh plasma and dried-blood-spot specimens

Sylvie Faucher1, Alexandre Martel, Alice Sherring

  • 1National HIV Immunology Laboratory, National HIV and Retrovirology Laboratories, Centre for Infectious Disease Prevention and Control, Health Canada, Ottawa, ON, Canada. sylvie_faucher@hc-sc.gc.ca

Clinical Chemistry
|July 2, 2004
PubMed
Abstract

No abstract available in PubMed .

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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.