Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two-color two-photon 4Pi fluorescence microscopy.

Jianfang Chen1, Katsumi Midorikawa

  • 1Laser Technology Laboratory, RIKEN, Hirosawa 2-1, Wako, Saitama 351-0198, Japan. chen@postman.riken.go.jp

Optics Letters
|July 6, 2004
PubMed
Summary

Combining two-color two-photon and 4Pi fluorescence microscopy overcomes sidelobe artifacts. This novel approach yields a single sharp fluorescence spot, enhancing imaging resolution and clarity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preserving bare mudflats reduces methane emissions: Implications for coastal wetland management.

Journal of environmental management·2026
Same author

Quantitative apportionment of multiple water sources and their implications for coastal nutrient dynamics: A mult-tracer framework of river-dominated system.

Water research·2026
Same author

Extreme drought-induced significant reduction of particulate organic carbon (POC) and its impact on apparent oxygen utilization (AOU) in the Changjiang Estuary and adjacent East China Sea.

Marine pollution bulletin·2026
Same author

The isolation, characterization, and bio-activity of two polysaccharides in <i>Rhodomyrtus tomentosa</i> (Aiton) Hassk. fruits.

Natural product research·2026
Same author

Correction: Chemoautotrophic Thermodesulfobacteriota as a key genomic potential group in the hypoxic diazotrophic community of the Changjiang (Yangtze River) estuary.

Frontiers in microbiology·2026
Same author

Identification of the Novel HLA-A*02:07:26 Allele Using PacBio Sequencing.

HLA·2025

Area of Science:

  • Optical microscopy
  • Fluorescence imaging
  • Super-resolution techniques

Background:

  • 4Pi fluorescence microscopy exhibits a point-spread function with a central lobe and interference sidelobes.
  • These sidelobes introduce artifacts, compromising image quality in high-resolution microscopy.

Purpose of the Study:

  • To address the sidelobe artifact issue in 4Pi fluorescence microscopy.
  • To enhance image fidelity and resolution by mitigating interference patterns.

Main Methods:

  • Proposed a hybrid approach combining two-color two-photon fluorescence microscopy with 4Pi fluorescence microscopy.
  • Utilized dual excitation wavelengths (400 nm and 800 nm) for excitation.
  • Simulated detection at a 350-nm emission wavelength.

Main Results:

  • Simulation results demonstrate the successful suppression of sidelobe artifacts.
  • Achieved a single, sharp fluorescence spot, indicating improved spatial resolution.
  • Validated the effectiveness of the combined microscopy technique.

Conclusions:

  • The integration of two-color two-photon and 4Pi fluorescence microscopy effectively overcomes sidelobe artifacts.
  • This method offers a promising strategy for generating high-resolution, artifact-free fluorescence images.
  • The proposed technique advances the capabilities of super-resolution fluorescence microscopy.

Related Experiment Videos