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Cell surface antigens expressed on polymorphonuclear cells in the peripheral blood from patients with inflammatory
K Mishima1, Y Ichikawa, H Shimizu
1Department of Internal Medicine, Tokai University School of Medicine, Kanagawa, Japan.
Abstract:
Cell surface antigens, such as CR3, Fc gamma RIII, cALLa and Leu 8 antigen, expressed on polymorphonuclear cells (PMN) in the peripheral blood (PB) are associated with certain functions of PMN. Functional alterations of PMN have been reported in some rheumatic diseases. In this study, therefore, the cell surface antigens were examined by 2-color flow-cytometry using monoclonal antibodies to clarify whether expressions of the antigens on PMN are abnormal in patients with rheumatic diseases including Behçet's disease (BD), rheumatoid arthritis (RA) and Sjögren's syndrome (SS). We found that expressions of CR3 and cALLa were increased on PB-PMN from patients with BD or RA, but not from those with SS. In BD, we further determined the phagocytic activity of PMN by a flow-cytometric method using fluorescent particles. The phagocytic activity was significantly higher in BD patients than in healthy controls, and correlated slightly with the expression of CR3 on PMN in the patients. In addition, prednisolone or colchicine seemed to decrease the expression of CR3 and/or cALLa in patients. The above results indicated that PB-PMN in BD and RA, but not SS, are phenotypically activated in vivo, and the activation probably reflects several cytokines and/or other stimuli to PMN produced in the inflammatory process.
Insights
Polymorphonuclear cell (PMN) surface antigens CR3 and cALLa are elevated in Behçet's disease (BD) and rheumatoid arthritis (RA) patients, indicating in vivo activation. This activation correlates with increased phagocytic activity in BD.
Area of Science:
- Immunology
- Rheumatology
- Cell Biology
Background:
- Polymorphonuclear cells (PMN) possess cell surface antigens crucial for their function.
- Functional alterations in PMN are observed in various rheumatic diseases.
- Specific antigens like CR3, Fc gamma RIII, cALLa, and Leu 8 are expressed on PMN.
Purpose of the Study:
- To investigate the expression of cell surface antigens on peripheral blood PMN in patients with Behçet's disease (BD), rheumatoid arthritis (RA), and Sjögren's syndrome (SS).
- To determine if these antigens are abnormally expressed in these rheumatic conditions.
- To assess the functional activity of PMN and its correlation with antigen expression in BD.
Main Methods:
- Two-color flow cytometry using monoclonal antibodies to analyze cell surface antigen expression on peripheral blood PMN.
- Flow cytometry-based assay using fluorescent particles to measure PMN phagocytic activity.
- Examination of antigen expression and phagocytic activity in patients with BD, RA, SS, and healthy controls.
Main Results:
- Increased expression of CR3 and cALLa antigens was observed on peripheral blood PMN from patients with BD and RA, but not SS.
- Phagocytic activity of PMN was significantly higher in BD patients compared to healthy controls.
- A slight correlation was found between CR3 expression and phagocytic activity in BD patients.
- Treatment with prednisolone or colchicine appeared to reduce CR3 and/or cALLa expression in patients.
Conclusions:
- Peripheral blood PMN in patients with BD and RA exhibit phenotypic activation in vivo.
- This in vivo activation of PMN is likely a response to inflammatory cytokines and stimuli present in these diseases.
- Sjögren's syndrome patients did not show similar PMN phenotypic alterations.
- The findings suggest a role for PMN activation in the pathogenesis of BD and RA.