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Transcriptomics and proteomics of human skin.

Bastiaan J H Jansen1, Joost Schalkwijk

  • 1Department of Tumour Immunology, Nijmegen Center for Molecular Life Sciences, University Medical Center, University of Nijmegen, The Netherlands. b.jansen@ncmls.kun.nl

Briefings in Functional Genomics & Proteomics
|July 9, 2004
PubMed
Summary

Large-scale gene expression analysis, including serial analysis of gene expression (SAGE) and microarrays, offers new insights into keratinocyte biology and skin diseases. This review covers current methods and their applications in understanding human skin.

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Area of Science:

  • Dermatology and Molecular Biology
  • Genomics and Transcriptomics

Background:

  • The epidermis serves as a protective barrier and environmental interface.
  • Keratinocytes within the epidermis express numerous genes crucial for skin function.
  • Recent advancements allow for large-scale analysis of keratinocyte gene expression.

Purpose of the Study:

  • To review the current state of large-scale gene expression analysis in human skin.
  • To emphasize the roles of serial analysis of gene expression (SAGE) and cDNA microarrays.
  • To discuss the merits and limitations of transcriptomics and proteomics.

Main Methods:

  • Transcriptome analysis: Serial Analysis of Gene Expression (SAGE) and microarrays.
  • Proteome analysis.
  • Utilizing intact human epidermis and in vitro keratinocyte models.

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Main Results:

  • Large-scale analyses provide deeper insights into keratinocyte biology.
  • These methods aid in understanding human skin diseases.
  • Comparison of transcriptomics and proteomics approaches.

Conclusions:

  • Large-scale gene expression analysis is vital for advancing skin biology and disease research.
  • SAGE and microarrays are key technologies in this field.
  • Careful consideration of methods and sample preparation is essential for reliable results.