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Human milk oligosaccharides reduce platelet-neutrophil complex formation leading to a decrease in neutrophil beta 2

Lars Bode1, Silvia Rudloff, Clemens Kunz

  • 1Institute of Nutritional Science, Justus-Liebig-University Giessen, Germany. Lbode@burnham.org

Journal of Leukocyte Biology
|July 9, 2004
PubMed
Summary
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Acidic human milk oligosaccharides (HMO) reduce platelet-neutrophil complex formation and neutrophil activation. These findings suggest HMO act as anti-inflammatory agents, potentially explaining lower infant inflammatory disease rates in breast-fed infants.

Area of Science:

  • Immunology
  • Nutritional Science
  • Pediatrics

Background:

  • Human milk oligosaccharides (HMO) are abundant in breast milk and possess structures similar to selectin ligands.
  • Platelet-neutrophil complexes (PNC) are involved in immune responses and disease pathogenesis.
  • The influence of HMO on PNC formation and neutrophil activation is not well understood.

Purpose of the Study:

  • To investigate the effect of HMO on the formation of platelet-neutrophil complexes (PNC).
  • To examine the impact of HMO on neutrophil activation.
  • To determine if acidic or neutral HMO fractions exhibit anti-inflammatory properties.

Main Methods:

  • Blood from healthy volunteers was treated with adenosine 5'-diphosphate to activate platelets.
  • Human milk oligosaccharides (HMO), standards, or control solutions were added to the blood samples.

Related Experiment Videos

  • Fluorescein-activated cell sorter analysis was used to quantify PNC formation and neutrophil activation markers (CD42a and CD11b).

    • Main Results:

    • An acidic HMO fraction significantly reduced PNC formation by up to 20% at physiological concentrations.
    • Associated neutrophils exhibited a dose-dependent decrease in beta 2 integrin expression (up to 30%) with acidic HMO.
    • Neutral HMO fractions demonstrated no significant effect on PNC formation or neutrophil activation.

    Conclusions:

    • Acidic HMO fractions possess anti-inflammatory properties by inhibiting PNC formation and neutrophil activation.
    • These findings support the role of acidic HMO as beneficial components of human milk.
    • HMO may contribute to the reduced incidence of inflammatory conditions like necrotizing enterocolitis in breast-fed infants.