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Cloning of cDNAs encoding multiple subtypes of feline interferon-alpha from the feline epitherial cell line
Ayuko Nagai1, Osamu Taira, Miyuki Ishikawa
1Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan.
Abstract:
Mammalian interferon (IFN)-alpha consists of a 23-amino acid signal peptide and a 166-amino acid mature protein. Feline (Fe) IFN-alpha has an extra unique molecule consisting of a 171-amino acid mature protein with a 5-amino acid insertion. We cloned eight new subtypes of cDNA encoding FeIFN- alpha from a feline epithelial cell line. Among all the FeIFN-alpha subtypes, including six that have previously been reported, the variations were found to be far less than those of IFN-alphas of other animals.
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