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Genetic dissection of corticosteroid receptor function in mice
T M Wintermantel1, S Berger, E F Greiner
1Molecular Biology of the Cell I, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|July 9, 2004
Summary
New mouse models with diverse corticosteroid receptor mutations offer insights into in vivo function. These models aid in identifying therapeutic compounds and understanding steroid signaling pathways for targeted interventions.
Area of Science:
- Genomics
- Molecular Biology
- Pharmacology
Background:
- Corticosteroid receptors play crucial roles in various physiological processes.
- Understanding their function in vivo is essential for developing targeted therapies.
- Existing mouse models may not fully capture the complexity of corticosteroid receptor signaling.
Purpose of the Study:
- To generate and characterize a comprehensive series of mouse models with diverse mutations in corticosteroid receptors.
- To utilize these models for a deeper molecular understanding of corticosteroid function in vivo.
- To facilitate the identification of novel therapeutic compounds targeting corticosteroid receptors.
Main Methods:
- Artificial chromosome-based transgenesis
- Conditional gene targeting
- Generation of allelic series for corticosteroid receptors (loss-of-function, gain-of-function, spatially/temporally restricted, tissue-specific, function-selective mutations)
Main Results:
- Successfully generated mouse models with a wide range of corticosteroid receptor mutations.
- Established a valuable resource for studying in vivo corticosteroid receptor function.
- These models enable the identification of steroid receptor target genes and signaling cascades.
Conclusions:
- The developed mouse models are instrumental for dissecting distinct corticosteroid receptor functions.
- These models support the discovery of tissue-specific and function-selective modulators of steroid receptors.
- The research provides a foundation for evaluating therapeutic interventions targeting corticosteroid signaling pathways.