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A classical live attenuated vaccine for sheep pox.
V Bhanuprakash1, B K Indrani, R Hegde
1Department of Veterinary Microbiology, Veterinary College, University of Agricultural Sciences, HA Farm, Hebbal, Bangalore, Karnataka, India. bhanupraaakash@yahoo.co.in
Tropical Animal Health and Production
|July 10, 2004
Summary
A live attenuated sheep pox vaccine was developed and proven safe and effective in sheep. Vaccinated animals demonstrated robust cell-mediated and humoral immunity against sheep pox virus (SPV).
Area of Science:
- Veterinary Virology
- Immunology
Background:
- Sheep pox virus (SPV) poses a significant threat to sheep populations worldwide.
- Development of effective and safe vaccines is crucial for controlling SPV outbreaks.
Purpose of the Study:
- To prepare and evaluate a classical live attenuated sheep pox vaccine using the Ranipet strain.
- To assess the safety and immunogenicity of the developed vaccine in sheep.
Main Methods:
- A live attenuated SPV vaccine was generated through 50 passages in a lamb testicular cell system.
- Vaccine safety was tested in laboratory animals, and efficacy was assessed in SPV-seronegative lambs.
- Field trial involved 660 sheep vaccinated with 1 x 10(2) TCID50; immune responses were monitored for 6 months.
Main Results:
- The vaccine exhibited high viral titers (TCID50: 10(9.63)/ml, RD50: 10(9.51)/ml).
- The vaccine was found to be safe with no adverse effects in laboratory animals and SPV-seronegative lambs.
- Vaccinated sheep showed significant cell-mediated (glucose utilization test) and humoral (serum neutralization test) immune responses.
Conclusions:
- The classical live attenuated sheep pox vaccine is safe and effective for sheep.
- The vaccine successfully induces both cell-mediated and humoral immunity, providing protection against sheep pox virus.