Related Experiment Videos
Quantification of desferrioxamine and its iron chelating metabolites by high-performance liquid chromatography and
S Singh1, N Mohammed, R Ackerman
1Chelsea Department of Pharmacy, King's College, University of London, United Kingdom.
Analytical Biochemistry
|May 15, 1992
Abstract:
An HPLC-based method for quantification of desferrioxamine (DFO) and its iron chelating metabolites in plasma has been developed. This assay overcomes stability problems associated with DFO by the addition of radioactive iron to convert unbound drug and metabolites to radio-iron-bound species. A dual detection system utilizing uv-vis absorption and radioactive (beta-particle) detector was used to quantify total and radio-iron-bound species. The use of octadecyl silanol solid phase extraction cartridges permits concentration of samples and allows accurate quantification of drug and metabolites down to 0.1 nmol/ml.