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Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

Protein arginine methylation during lytic adenovirus infection

Julia Kzhyshkowska1, Elisabeth Kremmer, Markus Hofmann

  • 1Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Landshuterstr. 22, D-93053 Regensburg, Germany. julia.kzhyshkowska@haut.ma.uni-heidelberg.de

The Biochemical Journal
|July 10, 2004
PubMed

Insights

Protein arginine methylation is crucial for adenovirus replication. This study shows viral proteins like E1B-AP5 and L4-100 kDa are methylated, enhancing viral production in host cells.

Area of Science:

  • Molecular Biology
  • Virology
  • Post-translational Modifications

Background:

  • Protein arginine methylation is a key post-translational modification impacting cellular processes.
  • Adenovirus (Ad) infection involves complex interactions with host cell machinery.
  • Previous work identified arginine methylation of Ad E1B-associated protein E1B-AP5.

Purpose of the Study:

  • To investigate protein arginine methylation in Ad-infected cells, focusing on E1B-AP5 and the viral L4-100 kDa protein.
  • To determine the role of host cell protein arginine methylation in Ad replication efficiency.
  • To analyze cell-type-specific methylation patterns of viral proteins.

Main Methods:

  • Analysis of protein arginine methylation in Ad-infected human cell lines (HeLa, MCF-7, H1299).
  • In vivo complex formation assays between viral proteins and methyltransferases.
  • In vitro methylation assays using purified HRMT1L2 (human protein arginine methyltransferase 1).

Main Results:

  • Arginine methylation of E1B-AP5 is enhanced during Ad infection in a cell-type-specific manner.
  • The viral L4-100 kDa protein is efficiently methylated in Ad-infected cells and forms complexes with methyltransferases in vivo.
  • L4-100 kDa can be methylated in vitro by HRMT1L2.
  • Correlation observed between protein arginine methylation profiles and Ad protein production efficiency.

Conclusions:

  • Protein arginine methylation of viral proteins is enhanced during adenovirus infection.
  • Host cell protein arginine methylation appears to be essential for efficient adenovirus replication.
  • Specific methyltransferases like HRMT1L2 may play a role in methylating viral proteins.

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