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Related Experiment Videos

PTTG/securin activates expression of p53 and modulates its function.

Tariq Hamid1, Sham S Kakar

  • 1Department of Medicine, University of Louisville, Louisville, KY 40202, USA. t0hami01@louisville.edu

Molecular Cancer
|July 10, 2004
PubMed
Summary

Pituitary tumor transforming gene (PTTG) activates p53 expression and function, indirectly influencing c-myc and Bax gene expression to promote apoptosis. This oncogene

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Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • Pituitary tumor transforming gene (PTTG) is an oncogene overexpressed in most tumors.
  • PTTG promotes cellular transformation and tumor formation.
  • PTTG's role in apoptosis and its relationship with p53 are not fully understood.

Purpose of the Study:

  • To investigate the effects of PTTG overexpression on p53 expression and function.
  • To elucidate the mechanisms by which PTTG influences p53 and downstream targets.
  • To determine PTTG's role in apoptosis induction.

Main Methods:

  • Overexpression of PTTG in HEK293 cells.
  • Analysis of p53 and c-myc gene expression at transcriptional and translational levels.
  • Deletion analysis of the p53 gene promoter.

Related Experiment Videos

  • Assessment of Bax gene expression and apoptosis induction.

    • Main Results:

    • PTTG overexpression regulates p53 expression both transcriptionally and translationally.
    • PTTG-mediated p53 activation is indirect, involving c-myc regulation.
    • PTTG stimulates Bax gene expression and induces apoptosis in HEK293 cells, mediated by p53 modulation.

    Conclusions:

    • PTTG overexpression activates p53 expression and modulates its function via c-myc regulation.
    • PTTG up-regulates Bax promoter activity and Bax expression through p53 modulation.
    • PTTG plays a significant role in apoptosis induction through the p53 pathway.