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Related Concept Videos

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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Related Experiment Video

Updated: Jul 15, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

Small RNAs shed some light.

Susan Gottesman1

  • 1Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.

Cell
|July 10, 2004
PubMed
Summary

Multiple small regulatory RNAs are crucial for controlling quorum sensing in Vibrio species. These small RNAs regulate gene expression and influence virulence in bacteria like Vibrio cholerae.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Small regulatory RNAs (sRNAs) are key players in bacterial gene regulation.
  • Quorum sensing (QS) is a cell-to-cell communication system controlling collective behaviors in bacteria.
  • Vibrio species, including Vibrio cholerae, utilize QS for various functions, including virulence.

Purpose of the Study:

  • To investigate the role of small regulatory RNAs in the quorum sensing systems of Vibrio species.
  • To identify specific sRNAs involved in regulating QS and virulence in Vibrio cholerae.

Main Methods:

  • Analysis of small RNA populations in Vibrio species.
  • Genetic manipulation to assess the function of identified sRNAs.
  • Assays to measure QS system activity and virulence factor production.

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Last Updated: Jul 15, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

AQRNA-seq for Quantifying Small RNAs
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AQRNA-seq for Quantifying Small RNAs

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Main Results:

  • Identification of multiple small regulatory RNAs essential for Vibrio quorum sensing.
  • Demonstration that these sRNAs directly regulate QS components.
  • Evidence for sRNA-mediated regulation of Vibrio cholerae virulence.

Conclusions:

  • Small regulatory RNAs are indispensable regulators of quorum sensing in Vibrio.
  • Targeted manipulation of sRNAs offers a potential strategy for controlling Vibrio virulence and QS-dependent processes.