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Related Experiment Videos

[Epithelial membrane antigen in lymphosarcoma cells (immunomorphologic study)].

A V Smirnov, N A Probatova, E N Sholokhova

    Arkhiv Patologii
    |January 1, 1992
    PubMed
    Summary
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    Immunohistochemistry for epithelial membrane antigen (EMA) can be misleading in diagnosing lymphosarcoma. A broader antibody panel, including vimentin and cytokeratin, is crucial for accurate differentiation from carcinoma.

    Area of Science:

    • Oncology
    • Immunohistochemistry
    • Pathology

    Context:

    • Distinguishing lymphosarcoma from poorly differentiated carcinoma is challenging.
    • Epithelial membrane antigen (EMA) is a commonly used marker, but its utility in lymphosarcoma diagnosis is debated.
    • New Soviet monoclonal antibodies (ICO-25) against EMA were included in the study.

    Purpose:

    • To evaluate the diagnostic utility of epithelial membrane antigen (EMA) and other markers in differentiating lymphosarcoma subtypes.
    • To investigate the binding of EMA, including ICO-25, to lymphosarcoma cells.
    • To assess the variability of vimentin staining in lymphosarcoma cells.

    Summary:

    • Eleven cases of T- and B-cell lymphosarcoma were studied using immunohistochemistry with antibodies against EMA (including ICO-25), common leucocytic antigen, vimentin, and cytokeratin.

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  • EMA and ICO-25 binding was observed in some lymphosarcoma cells, challenging its exclusive use as an epithelial marker.
  • Variable vimentin staining and other limitations highlight the need for a comprehensive antibody panel.

    • Impact:

    • The findings underscore that EMA alone is insufficient for differentiating lymphosarcoma from poorly differentiated carcinoma.
    • A spectrum of antibodies, including EMA, vimentin, cytokeratin, and common leucocytic antigen, is necessary for accurate diagnosis.
    • This research improves diagnostic accuracy in hematologic malignancies and solid tumors, impacting patient treatment strategies.