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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
[Chemical reactions in double-helical nucleic acids. XIV. Effectiveness of forming phosphodiester bonds between
Bioorganicheskaia Khimiia
|January 1, 1992
Abstract:
Efficiency of the template-directed condensation of oligonucleotides depends on the nature of the nucleotide units to be joined. Fourteen out of sixteen dinucleotide combinations in double-stranded DNA were examined. Alterations of the reaction efficiency are identical for cyanogen bromide and 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide coupling reagents. Dependence of the chemical ligation efficiency on the DNA sequence-specific local conformation is discussed.
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