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Influence of farnesyl transferase inhibitor treatment on epidermal growth factor receptor status
Bart Cornelissen1, Thomas Thonissen, Veerle Kersemans
1Laboratory of Radiopharmacy, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium. bart.cornelissen@ugent.be
Abstract:
The radiolabelled growth factor [(123/125)I] I-hEGF is evaluated in vitro and in vivo to monitor the acute effects on the EGFR of R115777, a farnesyl transferase inhibitor (FTI). Upregulation of the EGFR after incubation with R115777 correlated linearly with FTI induced acute growth inhibition. Receptor mediated [125I] I-hEGF internalization decreased following R115777 treatment. Preliminary data suggest that the net in vivo effect is a decrease of [123I] I-hEGF uptake in the tumour. These findings suggest the possible use of radioiodinated hEGF as a radiodiagnosticum to investigate EGFR status changes as a predictor for eventual FTI chemotherapy outcome in vivo.
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