Related Experiment Video
Updated: Aug 23, 2026
Cationic Nanoemulsion-Encapsulated Retinoic Acid as an Adjuvant to Promote OVA-Specific Systemic and Mucosal Responses
Published on: February 23, 2024
Immunogenicity in mice of a cationic microparticle-adsorbed plasmid DNA encoding Japanese encephalitis virus envelope
Rupinderjeet Kaur1, Manish Rauthan, Sudhanshu Vrati
1Virology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, JNU Complex, New Delhi 110067, India.
Abstract:
Previously, we described a plasmid pMEa, synthesizing the anchored form of Japanese encephalitis virus (JEV) envelope protein that generated virus-neutralizing antibodies in mice upon intra-muscular injection. The immunized mice showed significant protection against lethal JEV challenge. In order to improve the efficacy of plasmid DNA immunization against JEV, methods need to be employed that would enhance neutralizing antibody titers. Plasmid DNA adsorbed to cationic microparticles has recently been shown to significantly improve its immunogenicity. In the present study, we have adsorbed the plasmid pMEa on cationic microparticles and have compared its immunogenicity with the naked plasmid DNA. As seen in ELISA, the microparticle-adsorbed DNA induced higher titers of anti-JEV antibodies when compared to those induced by the naked DNA. No difference, however, was seen in JEV neutralization titers. The microparticle-adsorbed DNA induced a mixed Th1-Th2 kind of immune responses as opposed to Th1 type of immune responses elicited by the naked DNA.
Insights
Adsorbing Japanese encephalitis virus (JEV) DNA onto cationic microparticles enhanced antibody production compared to naked DNA. This method shows promise for improving DNA vaccine efficacy against JEV.
Area of Science:
- Immunology
- Virology
- Biotechnology
Background:
- Japanese encephalitis virus (JEV) poses a significant public health threat.
- Plasmid DNA vaccines offer a potential strategy for JEV prevention.
- Enhancing the immunogenicity of DNA vaccines is crucial for improved efficacy.
Purpose of the Study:
- To evaluate the immunogenicity of JEV DNA vaccine (pMEa) adsorbed onto cationic microparticles.
- To compare the immune responses elicited by microparticle-adsorbed DNA versus naked DNA.
- To assess the potential of microparticle-adsorbed DNA for enhancing JEV vaccine efficacy.
Main Methods:
- Adsorption of plasmid pMEa encoding JEV envelope protein onto cationic microparticles.
- Intramuscular immunization of mice with microparticle-adsorbed DNA and naked DNA.
- Quantification of anti-JEV antibody titers using ELISA.
- Assessment of JEV neutralization titers.
- Analysis of T-helper cell responses (Th1/Th2).
Main Results:
- Microparticle-adsorbed JEV DNA induced significantly higher anti-JEV antibody titers compared to naked DNA.
- No significant difference was observed in JEV neutralization titers between the two groups.
- Naked DNA elicited a Th1 immune response, while microparticle-adsorbed DNA induced a mixed Th1-Th2 response.
Conclusions:
- Cationic microparticles enhance the humoral immune response to JEV DNA vaccines.
- The mixed Th1-Th2 response induced by microparticle-adsorbed DNA may offer broader protection.
- Further studies are warranted to optimize microparticle-based JEV DNA vaccines for improved efficacy.
More Related Videos
13:36Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
Published on: May 6, 2015
09:37Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
Published on: September 23, 2022