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Updated: Aug 8, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Published on: May 1, 2013
Protein-DNA array-based identification of transcription factor activities regulated by interaction with the
Xin Jiang1, Michael Norman, Leslie Roth
1Panomics, Inc., Redwood City, California 94063 and LINE, Bristol University, Bristol BS1 3NY, United Kingdom. xjiang@panomics.com
Abstract:
The glucocorticoid receptor (GR) regulates gene expression by binding specific sequence elements within the promoters of target genes or by cross-talk with other transcription factors (TFs). For some TFs, interaction with the GR results in alteration of DNA binding and transcriptional regulation. We used a protein-DNA array, a system that facilitates simultaneous profiling of the activities of multiple transcription factors, to systematically examine the potential cross-talk of GRalpha with 149 TFs. Using this array, we identified several TFs, including IRF, E47, and COUP-TF, whose DNA binding activities were modulated by GRalpha. We then confirmed these results with in vitro electrophoretic mobility shift assays and in vivo reporter assays. In this study, IRF and E47 were identified as participants in GRalpha cross-talk for the first time. This new finding expands our understanding of the functional role of GRalpha in the context of gene expression regulation.
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