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Immobilization of immunoglobulin on a quartz surface by BrCN without loss of antigen binding capability

L D Krapivinskaya1, G B Krapivinsky, V L Ratner

  • 1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow.

Biosensors & Bioelectronics
|January 1, 1992
PubMed

BrCN-activated quartz was used for the immobilization of monoclonal immunoglobulins. BrCN-activated quartz keeps its ability to quantitatively bind immunoglobulins for at least 8 h. The surface density of the immobilized immunoglobulins was 100 ng cm-2 and they were found to retain their ability to bind antigen in a molar ratio of 1:2 for not less than three months.

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