Related Experiment Videos
LDL oxidative modifications in well- or moderately controlled type 2 diabetes
P G Scheffer1, R M A Henry, E J M Wever
1Metabolic Laboratory, Department of Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands. p.scheffer@vumc.nl
Diabetes/Metabolism Research and Reviews
|July 14, 2004
Summary
Type 2 diabetes increases in vivo oxidation of LDL, indicated by elevated ketocholesterol and Ox-apoB levels. This occurs even with well-controlled diabetes, suggesting increased oxidative stress in these patients.
Area of Science:
- Biochemistry
- Cardiovascular Medicine
- Endocrinology
Background:
- Type 2 diabetes is associated with increased oxidative stress.
- Low-density lipoprotein (LDL) oxidation is implicated in cardiovascular disease development.
- Carotid intima-media thickness (IMT) is a marker of atherosclerosis.
Purpose of the Study:
- To investigate in vivo LDL oxidation in type 2 diabetic patients.
- To assess the association between LDL oxidation and carotid IMT.
- To identify specific markers of LDL oxidation elevated in type 2 diabetes.
Main Methods:
- Quantified five LDL oxidation products in 38 type 2 diabetic patients and 38 controls.
- Measured baseline conjugated dienes (BCD), 7-OH-glycero-phosphocholine (7-OH-GPC), lyso-phosphatidylcholine (lyso-PC), and ketocholesterol in LDL.
- Assessed circulating in vivo oxidized apolipoprotein B100 (Ox-apoB) and carotid IMT via ultrasound.
Main Results:
- LDL-ketocholesterol and Ox-apoB were significantly increased in diabetic patients (p < 0.05).
- Levels of BCD, 7-OH-GPC, and lyso-PC did not differ between groups.
- Carotid IMT showed borderline higher values in diabetic individuals (p = 0.06) with no significant correlation to LDL oxidation markers.
Conclusions:
- Stable end products of LDL oxidation, ketocholesterol and Ox-apoB, are elevated in type 2 diabetes.
- In vivo LDL oxidation is increased in type 2 diabetic patients, even with moderate glycemic control.
- Intermediary LDL oxidation products were not significantly different, suggesting a specific pathway of oxidation.