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Related Experiment Videos

Desmin expression during early mouse tongue morphogenesis.

M L Mayo1, P Bringas, V Santos

  • 1Center for Craniofacial Molecular Biology, University of Southern California, School of Dentistry, Los Angeles 90033.

The International Journal of Developmental Biology
|June 11, 1992
PubMed
Summary
This summary is machine-generated.

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Occipital somites are crucial for tongue muscle development, with cells migrating to form the tongue. Early mouse tongue development and muscle progenitor cell expansion occur in vitro without serum factors.

Area of Science:

  • Developmental Biology
  • Muscle Biology
  • Craniofacial Development

Background:

  • Occipital somites supply progenitor cells for craniofacial muscles, including the tongue.
  • Serum factors are generally considered essential for in vitro myogenesis.
  • Previous research has not fully elucidated the role of serum in early tongue development.

Purpose of the Study:

  • To investigate early mouse tongue development in a serumless, chemically-defined medium.
  • To determine the expression and localization of desmin during embryonic mouse first branchial arch development.
  • To assess the necessity of serum-derived factors for in vitro myogenesis in tongue progenitor cells.

Main Methods:

  • Culture of embryonic mouse first branchial arch explants in serumless medium.

Related Experiment Videos

  • Immunohistochemical analysis to detect desmin expression.
  • Computer-assisted three-dimensional reconstructions to map cell migration pathways.
  • Quantification of desmin-expressing cells in cultured explants.

    • Main Results:

    • Desmin expression was observed in occipital somites at embryonic day 9 (E9).
    • A distinct cell migration pathway from somites to the tongue primordium was identified.
    • In vitro culture supported tongue morphogenesis, including the fusion of lateral lingual processes.
    • A significant five-fold increase in desmin-expressing cells was noted in E10 explants after 9 days of culture, despite lack of myotube fusion.

    Conclusions:

    • The tongue myogenic cell lineage is determined between E8 and E11.
    • Tongue progenitor cells can expand in number within explants cultured in serumless medium.
    • Serum is not an absolute requirement for the expansion of progenitor myoblasts or potential differentiation of other cell types into myoblasts during early tongue development.