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Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric
Yoko Sekiguchi1, Naoto Mitsuhashi, Yoshinori Inoue
1Department of Biological Sciences, Faculty of Science, Nara Women's University, Kitauoyanishimachi, Nara City, Nara 630-8506, Japan. sekiguchi@dionex.co.jp
Abstract:
This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD, a column packed with titanium dioxide resin was used. The titanium dioxide column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve.
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