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Updated: Aug 23, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
Published on: November 1, 2017
Fourier-domain holography in photorefractive quantum-well films
Kwan Jeong1, Leilei Peng, David D Nolte
1Purdue University, West Lafayette, Indiana 47907, USA. jeongk@physics.purdue.edu
Abstract:
Fourier-domain holography (FDH) is investigated as a candidate for holographic optical coherence imaging to produce real-time images of structure inside living tissue and turbid media. The effects of spatial filtering, the background intensity distributions, and the role of background noise in determining dynamic range are evaluated for both FDH and image-domain holography (IDH). The grating washout effect in FDH (edge enhancement) is removed by use of a vibrating diffuser that consequently improves the image quality. By comparing holographic images and background images of FDH and IDH we show that FDH provides a higher dynamic range and a higher image quality than IDH for this specific application of imaging diffuse volumetric objects.
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