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Related Experiment Video

Updated: Jul 17, 2026

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

A spurious markedly increased serum estradiol level due to an IgA lambda.

D L Gordon¹, E Holmes, E J Kovacs

¹Division of Endocrinology, Department of Medicine, Loyola University Medical Center, Maywood, Illinois, USA.

Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists

|July 15, 2004

Summary

Physicians should be aware of potential errors in hormone testing. A case showed a falsely high estradiol level due to an antibody interference, leading to unnecessary surgery.

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Last Updated: Jul 17, 2026

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Area of Science:

Clinical Chemistry
Endocrinology
Laboratory Medicine

Background:

Hormone level testing is crucial for diagnosing endocrine disorders.
Laboratory assays can sometimes yield erroneous results, impacting clinical decisions.

Purpose of the Study:

To highlight the potential for inaccurate hormone level measurements.
To emphasize the importance of correlating lab results with the clinical presentation.

Main Methods:

Case report of a patient with significantly elevated serum estradiol (E2).
Review of literature to understand the discrepancy between laboratory findings and clinical status.

Main Results:

A 41-year-old woman underwent unnecessary surgery due to a falsely elevated E2 level.
The elevated E2 was caused by IgA lambda interference with the radioimmunoassay's radiolabeled tracer.

Conclusions:

Discrepancies between repeated abnormal hormone levels and clinical findings warrant further investigation.
Consideration of alternative assay methods is recommended when assay interference is suspected.