[Characteristics of chromatin fibers immobilized on a polycationic surface]

The possibility of chromatin fiber immobilization on a polycationic surface formed by lysine-rich histones was investigated. The amount of lysine-rich histones preimmobilized on a hydrophobic surface was estimated. It was shown that depending on the initial solution concentration the number of immobilized fibers can reach a level characteristic of a bilayer package. Immobilized fibers preserve a nucleosomal structure and shown an ability in compactization at physiological values of the ionic strength. Possible applications of the described approach as a model system for the study of the chromatin domain organization is discussed.