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Updated: Aug 15, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
KeePin' the p53 family in good shape
Fiamma Mantovani1, Monica Gostissa, Licio Collavin
1Laboratorio Nazionale C.I.B., Area Science Park, Trieste, Italy.
Cell Cycle (Georgetown, Tex.)
|July 16, 2004
Summary
The prolyl isomerase Pin1 regulates cell proliferation and DNA damage response. Pin1 is now shown to be essential for the apoptotic activity of the p53 sibling protein, p73.
Area of Science:
- Molecular Biology
- Cell Biology
- Cancer Research
Background:
- The tumor suppressor p53 and prolyl isomerase Pin1 are key regulators of cell proliferation and transformation.
- Pin1 catalyzes conformational changes in phosphorylated proteins, influencing cell cycle, transcription, and differentiation.
- Pin1 modulates p53 function in DNA damage response.
Purpose of the Study:
- To investigate the role of Pin1 in the function of the p53 sibling protein, p73.
- To elucidate the regulatory mechanisms controlling p73-mediated apoptosis.
Main Methods:
- Western blotting
- Immunoprecipitation
- Cell viability assays
- Apoptosis assays
Main Results:
- Pin1 interacts with p73.
- Pin1 is required for p73-induced apoptosis.
- Pin1 enhances the apoptotic activity of p73.
Conclusions:
- Pin1 plays a crucial role in p73-mediated apoptosis.
- Pin1 represents a potential therapeutic target for enhancing cancer treatment strategies.
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