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Response related enzymatic changes in ovaries of superovulated mice
Ranjna S Cheema1, C K Dhanju, J S Matharoo
1Department of Animal Breeding and Genetics, College of Veterinary Science, Punjab Agricultural University, Ludhiana 141004, India.
Abstract:
Adult female mice were superovulated with PMSG followed by HCG and 140 blastocysts and 69 morulae were recovered from 24 mice. On the basis of the response, mice were divided into six groups; non responders, 1-5, 6-10, 11-20, 21-30 and >30 embryos. The ovaries of the animals were pooled group wise, homogenized in PBS (pH 7.4) and after centrifugation for 10-15 minutes, the supernatant was analyzed for the enzymes, guanine oxaloacetate transaminase (GOT), guanine pymvate transaminase (GPT), acid phosphatases (ACP) and alkaline phosphatases (AKP). Acid and alkaline phosphatase activities did not show any variation in relation to response to superovulation but GOT and GPT showed significantly increased activity in response to induction of superovulation. A statistically significant positive correlation was found between GOT and GPT activities and the superovulatory response in mice.
Insights
Superovulation induction in female mice significantly increased guanine oxaloacetate transaminase (GOT) and guanine pyruvate transaminase (GPT) enzyme activities. Higher GOT and GPT levels correlated positively with a greater superovulatory response, indicating their potential as biomarkers.
Area of Science:
- Reproductive Biology
- Biochemistry
- Animal Science
Background:
- Superovulation is a key technique in assisted reproductive technologies.
- Understanding the biochemical changes associated with superovulation is crucial for optimizing protocols.
- Enzyme activity in ovarian tissue may reflect the physiological response to hormonal stimulation.
Purpose of the Study:
- To investigate the relationship between ovarian enzyme activities and superovulatory response in adult female mice.
- To determine if specific enzymes, such as GOT, GPT, ACP, and AKP, correlate with the number of embryos recovered after superovulation induction.
Main Methods:
- Adult female mice were induced to superovulate using pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG).
- Ovaries were collected and grouped based on the number of embryos recovered (non-responders to >30 embryos).
- Ovarian homogenates were analyzed for guanine oxaloacetate transaminase (GOT), guanine pyruvate transaminase (GPT), acid phosphatase (ACP), and alkaline phosphatase (AKP) activities.
Main Results:
- Acid and alkaline phosphatase activities showed no significant variation across different superovulatory response groups.
- Both GOT and GPT activities were significantly increased in response to superovulation induction.
- A statistically significant positive correlation was observed between GOT and GPT activities and the superovulatory response.
Conclusions:
- GOT and GPT enzyme activities are elevated following superovulation induction in mice.
- Increased GOT and GPT levels are positively correlated with a higher superovulatory response.
- These enzymes may serve as potential biochemical markers for assessing superovulation efficiency in mice.
