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Sera from patients with idiopathic dilated cardiomyopathy decrease ICa in cardiomyocytes isolated from rabbits.

Cristiane Del Corsso1, Antônio Carlos Campos de Carvalho, Helena Furtado Martino

  • 1Department of Physiology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil. cdelcors@aecom.yu.edu

American Journal of Physiology. Heart and Circulatory Physiology
|July 17, 2004
PubMed
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Idiopathic dilated cardiomyopathy (IDC) patients possess autoantibodies targeting muscarinic M2 receptors. These antibodies act like agonists, potentially triggering arrhythmias and sudden cardiac death in IDC patients.

Area of Science:

  • Cardiology
  • Immunology
  • Electrophysiology

Background:

  • Autoantibodies against muscarinic and adrenergic receptors are found in idiopathic dilated cardiomyopathy (IDC) and Chagas disease.
  • The functional effects of these autoantibodies on cardiac receptors remain unclear.

Purpose of the Study:

  • To detect autoantibodies against muscarinic M2 and beta1-adrenergic receptors in IDC patients' sera.
  • To characterize the electrophysiological effects of these autoantibodies on rabbit cardiomyocytes.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) to detect autoantibodies.
  • Whole-cell patch-clamp technique to assess electrophysiological effects on rabbit ventricular myocytes.

Main Results:

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  • High titers of anti-muscarinic M2 receptor antibodies were detected in all IDC patients.
  • Antibodies against the beta1-receptor were found in 50% of IDC patients.
  • IDC patient sera significantly decreased isoproterenol-stimulated L-type Ca2+ currents (26%) and action potential duration (10.5%) in rabbit cardiomyocytes, mimicking agonist-like effects.

    • Conclusions:

    • Sera from IDC patients contain autoantibodies that functionally interact with muscarinic M2 receptors on cardiomyocytes.
    • These autoantibodies act in an agonist-like manner, altering cardiac electrogenesis.
    • This mechanism may contribute to ventricular arrhythmias and sudden death in IDC patients.