Related Experiment Videos
Thrombin generation assays: accruing clinical relevance
H Coenraad Hemker1, Raed Al Dieri, Suzette Béguin
1Synapse BV, Cardiovascular Research Institute, 6200 MD Maastricht, The Netherlands. H.C.Hemker@Thrombin.com
Current Opinion in Hematology
|July 17, 2004
Summary
Thrombin generation testing in platelet-poor plasma (PPP) and platelet-rich plasma (PRP) offers a comprehensive functional assessment of coagulation. This revived method aids in diagnosing various hemostatic disorders and monitoring therapies.
Area of Science:
- Biochemistry
- Hematology
- Clinical Diagnostics
Background:
- Thrombin generation, a key process in hemostasis, was historically underutilized as a diagnostic tool.
- Recent advancements have revitalized thrombin generation as a functional assay for the plasma coagulation system.
Purpose of the Study:
- To highlight the revival of thrombin generation as a functional test for plasma coagulation.
- To emphasize its utility in both platelet-poor plasma (PPP) and platelet-rich plasma (PRP).
Main Methods:
- Utilizing special fluorogenic thrombin substrates for real-time monitoring of thrombin concentration.
- Analyzing thrombin generation in clotting PPP and PRP across multiple parallel samples.
- Investigating cell-bound thrombin generation, particularly in PRP, using model systems.
Main Results:
- Fluorogenic substrates enable on-line monitoring of thrombin generation in up to 24 samples.
- Studies underscore the significance of cell-bound thrombin generation measured in PRP.
- The method successfully monitors thrombin concentration in both PPP and PRP.
Conclusions:
- Applicable to controlling heparin therapy and detecting lupus anticoagulant.
- Useful for identifying thrombomodulin and activated protein C resistance, including effects of oral contraceptives.
- Essential for monitoring hemophilia treatment and assessing platelet-related disorders like von Willebrand disease and Glanzmann and Bernard-Soulier thrombopathy, as well as antiplatelet drug efficacy.