CD14 C(-260)T gene polymorphism, circulating soluble CD14 levels and arteriosclerosis

Area of Science:

• Cardiovascular Science
• Genetics
• Immunology

Background:

• The CD14 pathway links infection and inflammation.
• Previous studies show conflicting results on CD14 C260T polymorphism, soluble CD14 (sCD14) levels, and atherosclerosis.
• Investigating these relationships in a population-based sample is crucial.

Purpose of the Study:

• To examine the association between CD14 C260T gene polymorphism, sCD14 blood levels, and arterial wall status.
• To determine if CD14 C260T polymorphism correlates with early atherosclerosis markers like intima-media thickness and plaque presence.

Main Methods:

• Analysis of 899 subjects from the Toulouse MONICA center with complete data.
• Measurement of sCD14 levels using an immuno-enzymatic method.
• Assessment of carotid intima-media thickness (IMT) and arterial plaques via ultrasonography, alongside genotypic analysis for CD14 C260T polymorphism.

Main Results:

• Subjects with the 't' allele showed increased sCD14 expression (P < 0.01).
• No significant association was found between the CD14 C260T polymorphism and IMT, plaque presence, or pulse wave velocity.
• A significant interaction (P < 0.05) between the C260T polymorphism and smoking was observed, where the 't' allele did not affect sCD14 levels in current smokers.

Conclusions:

• The CD14 C260T polymorphism is associated with sCD14 expression but not with early atherosclerosis markers in this population.
• This polymorphism influences plasma sCD14 levels, with an interaction observed concerning current smoking status.
• Further research is warranted to elucidate the role of this interaction in smoking-related vascular events.