Related Experiment Video
Updated: Aug 23, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Increased hepcidin expression and hypoferraemia associated with an acute phase response are not affected by
David M Frazer1, Sarah J Wilkins, Kirstin N Millard
1Iron Metabolism Laboratory, The Queensland Institute of Medical Research and The University of Queensland, PO Royal Brisbane Hospital, Brisbane, Queensland, Australia.
Abstract:
The effect of HFE inactivation on iron homeostasis during an acute phase response was investigated in mice. HFE knockout, beta2-microglobulin knockout and C57BL/6J mice were injected with Freund's Complete Adjuvant to induce an acute phase response and hepatic hepcidin expression and serum transferrin saturation was determined 16 h later. Hepcidin mRNA increased in all strains in response to an acute phase stimulus when compared with untreated control animals. Hypoferraemia also occurred in all strains, indicating that both the upregulation of hepcidin and the decrease in transferrin saturation associated with an acute phase response is not dependent on HFE function.
Related Concept Videos
Hepatitis
Regulation of Hematopoietic Stem Cells