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Updated: May 6, 2026
08:18
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
Published on: December 31, 2014
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Dominant negative MYC blocks transformation by ABL oncogenes
C L Sawyers1, W Callahan, O N Witte
1Department of Medicine, Howard Hughes Medical Institute, University of California, Los Angeles 90024.
Cell
|September 18, 1992
Summary
High MYC levels synergize with ABL oncogenes. Dominant-negative MYC suppressed ABL-induced transformation, demonstrating c-MYC
Area of Science:
- Oncogenomics
- Cellular Transformation
- Molecular Biology
Background:
- MYC oncogene expression often synergizes with ABL oncogenes.
- The precise role of MYC in ABL-mediated transformation requires further elucidation.
Purpose of the Study:
- To investigate whether MYC is essential for ABL oncogene-driven cellular transformation.
- To determine if MYC complements or is indispensable for ABL oncogene transformation.
Main Methods:
- Overexpression of dominant-negative MYC proteins in fibroblasts.
- Assessing the impact of dominant-negative MYC on transformation induced by v-abl and BCR-ABL oncogenes.
- Evaluating transformation of primary mouse bone marrow pre-B cells using retroviral constructs co-expressing BCR-ABL and MYC.
Main Results:
- Dominant-negative MYC reduced transformation by v-abl and BCR-ABL oncogenes 5- to 10-fold.
- Transformation induced by the serine/threonine kinase oncogene v-mos remained unaffected.
- Dominant-negative MYC suppressed BCR-ABL-mediated transformation of primary mouse bone marrow pre-B cells.
Conclusions:
- c-MYC is essential for ABL oncogene-mediated cellular transformation.
- These findings help define the molecular pathway involving MYC and ABL in oncogenesis.
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