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Updated: Aug 16, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Spectral and temporal speckle field measurements of a random medium
M A Webster1, T D Gerke, A M Weiner
1School of Electrical and Computer Engineering, Purdue University, 465 Northwestern Avenue, West Lafayette, Indiana 47907-2035, USA. webb@purdue.edu
Abstract:
The zero-mean circular complex Gaussian field statistics of a random medium are experimentally demonstrated in the optical domain, thus verifying this key assumption of statistical optics. Using a frequency-tunable laser source in a fixed-path-length interferometer, we obtain optical field fluctuations in the time and frequency domains that clearly show that the ensemble-averaged temporal intensity converges to the photon transit time distribution, which for the samples used is in excellent agreement with a diffusion model.

