Expression of c-Ki-ras in developing rat liver

1. Two major c-Ki-ras transcripts are present in rat liver throughout development. 2. Both transcripts are functional, i.e. translatable into ras protein. 3. They exhibit low levels in early foetuses and increase gradually towards term. 4. Peak levels of transcripts are detected on the fourth day of postnatal life. 5. Results from nuclear run-off assays demonstrate that the rise in level of transcripts is a result of increase in rate of transcription with the highest rate detected on the fourth day of postnatal life. 6. Western blot analysis reveals that the accumulation of c-Ki-ras protein clearly follows the pattern of changes in transcription of c-Ki-ras gene.