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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Restricted diffusion within a single pore
M E Hayden1, G Archibald, K M Gilbert
1Department of Physics, Simon Fraser University, Burnaby, BC, Canada V5A 1S6. mhayden@sfu.ca
Abstract:
The time-dependent diffusion of 3Heatoms perpendicular to the axis of a single macroscopically large cylindrical pore is studied using a steady (or constant) gradient-recalled echo sequence. Measurements of the effective 3Hediffusion coefficient extending from the free-diffusion regime to the motionally averaged regime are presented, and are well-described by analytic solutions to the Bloch-Torrey equation based on the gaussian phase approximation. Our data yield the value 0.140(6)m2/s for the self diffusion coefficient of 3Heat a temperature of 296 K and a pressure of 1.00 Torr. Adaptations of these methods should enable the study of complex pore geometries as model systems.
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