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Updated: Aug 23, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
Diaryl-substituted salicyl- and anthranyl-ketoximes as potential estrogen receptor ligands
Filippo Minutolo1, Michela Antonello, Simone Bertini
1Dipartimento di Scienze Farmaceutiche, Università di Pisa, Via Bonanno 6, 56126 Pisa, Italy.
Abstract:
3,4-diphenylsalicylaldoxime and 3,4-diphenylanthranylaldoxime derivatives, containing small groups (methyl or ethyl) on the imine carbon atom, were synthesized and submitted to biological assays. Binding tests performed on uterine cytosol estrogen receptor (ER) preparation and on purified full-length human ERalpha and ERbeta, showed that the newly synthesised compounds exhibit considerably lower binding affinities with respect to reference non-substituted 3,4-salicylaldoxime.
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