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Updated: Aug 23, 2026

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
Sequence analyses of cyanobacterial bicarbonate transporters and their homologues
Torsten von Rozycki1, Mark A Schultzel, Milton H Saier
1Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116, USA.
Abstract:
The primary HCO3- uptake system in the cyanobacterium Synecocystis is the Na+-dependent transporter SbtA. SbtA and its homologues were identified and shown to display a common topology of ten transmembrane segments (TMSs). These proved to have arisen by an intragenic duplication event from an ancestral gene encoding a five TMS protein product. A region of SbtA shows sufficient similarity to 10 TMS ABC-type integral membrane transport proteins to suggest a common origin. Phylogenetic analyses of the SbtA family revealed two clusters of cyanobacterial homologues with all non-cyanobacterial family members outside of these two clusters. The tree topology suggests that SbtA family members display multiple transport functions.
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