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Protein crystallization in the structural genomics era.

Alexander McPherson1

  • 1University of Califonia, Irvine, Department of Molecular Biology and Biochemistry, 560 Steinhaus Hall, Irvine, CA 92697-3900, USA. amcphers@uci.edu

Journal of Structural and Functional Genomics
|July 21, 2004
PubMed
Summary

Advances in macromolecular crystallization, driven by structural genomics, include physical studies, new methods, protein engineering, novel screening conditions, and automation. These developments accelerate protein structure determination.

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Area of Science:

  • Biochemistry and structural biology
  • Protein crystallization techniques

Background:

  • The field of macromolecular crystallization has seen significant progress in the last decade.
  • These advances are largely attributed to the international structural genomics initiative.

Purpose of the Study:

  • To summarize recent progress in macromolecular crystallization.
  • To highlight the influence of structural genomics on these advancements.

Main Methods:

  • Physical studies and characterization of protein crystallization.
  • Development of novel crystallization approaches and procedures.
  • Genetic protein engineering for enhanced purification and crystallization.
  • Creation of new screening conditions utilizing structural genomics data.

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  • Implementation of automation, robotics, and high-throughput screening.

    • Main Results:

    • Noteworthy advances have occurred across five key areas of macromolecular crystallization.
    • Significant progress has been concentrated in the last two years, coinciding with structural genomics outcomes.
    • Automation and robotics have enabled mass screening with minimal protein quantities.

    Conclusions:

    • The structural genomics initiative has profoundly impacted macromolecular crystallization.
    • These advancements are crucial for accelerating the determination of protein structures.
    • Future research will likely build upon these integrated approaches.